A. Sensitize a 96-well microtiter plate with purified antigen.
1. Prepare a solution of the purified antigen of interest in phosphate buffer (see recipe below) such that a concentration of approximately 10 µg/ml is achieved. Pipette 100 µl of this solution to all wells of the plate with the exception of those in the outermost rows and columns (these areas of the plate are prone to evaporation).
2. Cover the plate with Parafilm and incubate for 2 hours at 37°C, followed by an overnight incubation at 4°C. Cover the plate tightly to avoid evaporation. Store at 4°C. The plate(s) will remain usable for about one month.
B. Block the sensitized plate.
1. When ready to use, discard the antigen solution contained in the wells of the sensitized plate. Pat the inverted plate sharply several times against a pad of paper towels to insure complete removal of the solution.
2. Wash the wells three times with 1x PBS/0.05%-Tween 20. Pat to dry completely after the final wash, as above.
3. Pipette 100 µl of 5% skim milk/PBS/0.005% thimerasol (added to retard bacterial growth) to each well. Incubate the plate, covered with Parafilm, at 37°C for 2 hours to overnight. It is convenient during this incubation step to begin preparation of the samples to be tested, outlined below.
4. Wash the wells three times with PBS/Tween, dry as before after the last wash.
人mRNA加帽蛋白增强子1(EDC1)酶**测试剂盒(ELISA试剂盒) Homo sapiens (Human) Enhancer Of mRNA Decapping Protein 1 (EDC1)ELISA KIT
保质期：6个月， 我公司试剂盒系列产品均为**批次，请放心购买！如需要说明书请联系在线客服
操作注意事项
许多医院在使用酶标仪之前是通过目测判断结果，操作过程随意性较大，在使用酶标仪后操作必须规范。
A. 使用移液器加液，移液枪头不能混用。
B. 洗板要干净，避免交叉污染。
C. 严格按照试剂盒的说明书操作，反应时间准确。
D. 请勿将样品或试剂洒到仪器表面或内部，操作完成注意做好清洁工作。
E. 不要在测量过程中关闭电源。
F. 对于因试剂盒问题造成的测量结果的偏差，应根据实际情况及时修改参数，以达到**效果。
G. 使用后盖好防尘罩。
H. 出现技术故障时应及时与厂家联系，切勿擅自拆卸酶标仪。
人mRNA加帽蛋白增强子1(EDC1)酶**测试剂盒(ELISA试剂盒) Homo sapiens (Human) Enhancer Of mRNA Decapping Protein 1 (EDC1)ELISA KIT
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牛骨成型蛋白受体1A(BMPR1A)酶**测试剂盒(ELISA试剂盒) Bos taurus; Bovine (Cattle) Bone Morphogenetic Protein Receptor 1A (BMPR1A)ELISA KIT
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