A. Sensitize a 96-well microtiter plate with purified antigen.
1. Prepare a solution of the purified antigen of interest in phosphate buffer (see recipe below) such that a concentration of approximately 10 µg/ml is achieved. Pipette 100 µl of this solution to all wells of the plate with the exception of those in the outermost rows and columns (these areas of the plate are prone to evaporation).
2. Cover the plate with Parafilm and incubate for 2 hours at 37°C, followed by an overnight incubation at 4°C. Cover the plate tightly to avoid evaporation. Store at 4°C. The plate(s) will remain usable for about one month.
B. Block the sensitized plate.
1. When ready to use, discard the antigen solution contained in the wells of the sensitized plate. Pat the inverted plate sharply several times against a pad of paper towels to insure complete removal of the solution.
2. Wash the wells three times with 1x PBS/0.05%-Tween 20. Pat to dry completely after the final wash, as above.
3. Pipette 100 µl of 5% skim milk/PBS/0.005% thimerasol (added to retard bacterial growth) to each well. Incubate the plate, covered with Parafilm, at 37°C for 2 hours to overnight. It is convenient during this incubation step to begin preparation of the samples to be tested, outlined below.
4. Wash the wells three times with PBS/Tween, dry as before after the last wash.
猪补体成分3a(C3a)酶**测试剂盒(ELISA试剂盒) Sus scrofa; Porcine (Pig) Complement Component 3a (C3a)ELISA KIT
人Ig样域蛋白粘附分子1(AMIGO1)酶**测试剂盒(ELISA试剂盒) Homo sapiens (Human) Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1)ELISA KIT
大鼠唾液淀粉酶α1(AMY1)酶**测试剂盒(ELISA试剂盒) Rattus norvegicus (Rat) Amylase Alpha 1, Salivary (AMY1)ELISA KIT
人PP2A癌症抑制抑制因子(CIP2A)酶**测试剂盒(ELISA试剂盒) Homo sapiens (Human) Cancerous Inhibitor Of PP2A (CIP2A)ELISA KIT
人mRNA加帽蛋白增强子1(EDC1)酶**测试剂盒(ELISA试剂盒) Homo sapiens (Human) Enhancer Of mRNA Decapping Protein 1 (EDC1)ELISA KIT
人甲硫氨酸亚砜还原酶A(MSRA)酶**测试剂盒(ELISA试剂盒) Homo sapiens (Human) Methionine Sulfoxide Reductase A (MSRA)ELISA KIT
牛骨成型蛋白2(BMP2)酶**测试剂盒(ELISA试剂盒) Bos taurus; Bovine (Cattle) Bone Morphogenetic Protein 2 (BMP2)ELISA KIT
小鼠Midline 1蛋白(MID1)酶**测试剂盒(ELISA试剂盒) Mus musculus (Mouse) Midline 1 (MID1)ELISA KIT
牛骨成型蛋白受体1A(BMPR1A)酶**测试剂盒(ELISA试剂盒) Bos taurus; Bovine (Cattle) Bone Morphogenetic Protein Receptor 1A (BMPR1A)ELISA KIT
大鼠透明带糖蛋白2(ZP2)酶**测试剂盒(ELISA试剂盒) Rattus norvegicus (Rat) Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2)ELISA KIT
猪补体成分3a(C3a)酶**测试剂盒(ELISA试剂盒) Sus scrofa; Porcine (Pig) Complement Component 3a (C3a)ELISA KIT
人早老素1(PSEN1)酶**测试剂盒(ELISA试剂盒) Homo sapiens (Human) Presenilin 1 (PSEN1)ELISA KIT
豚鼠胰岛素(INS)酶**测试剂盒(ELISA试剂盒) Cavia (Guinea pig ) Insulin (INS)ELISA KIT
猪补体成分3a(C3a)酶**测试剂盒(ELISA试剂盒) Sus scrofa; Porcine (Pig) Complement Component 3a (C3a)ELISA KIT
