A. Sensitize a 96-well microtiter plate with purified antigen.
1. Prepare a solution of the purified antigen of interest in phosphate buffer (see recipe below) such that a concentration of approximately 10 µg/ml is achieved. Pipette 100 µl of this solution to all wells of the plate with the exception of those in the outermost rows and columns (these areas of the plate are prone to evaporation).
2. Cover the plate with Parafilm and incubate for 2 hours at 37°C, followed by an overnight incubation at 4°C. Cover the plate tightly to avoid evaporation. Store at 4°C. The plate(s) will remain usable for about one month.
B. Block the sensitized plate.
1. When ready to use, discard the antigen solution contained in the wells of the sensitized plate. Pat the inverted plate sharply several times against a pad of paper towels to insure complete removal of the solution.
2. Wash the wells three times with 1x PBS/0.05%-Tween 20. Pat to dry completely after the final wash, as above.
3. Pipette 100 µl of 5% skim milk/PBS/0.005% thimerasol (added to retard bacterial growth) to each well. Incubate the plate, covered with Parafilm, at 37°C for 2 hours to overnight. It is convenient during this incubation step to begin preparation of the samples to be tested, outlined below.
4. Wash the wells three times with PBS/Tween, dry as before after the last wash.
豚鼠胰岛素(INS)酶**测试剂盒(ELISA试剂盒) Cavia (Guinea pig ) Insulin (INS)ELISA KIT
牛骨成型蛋白受体1A(BMPR1A)酶**测试剂盒(ELISA试剂盒) Bos taurus; Bovine (Cattle) Bone Morphogenetic Protein Receptor 1A (BMPR1A)ELISA KIT
大鼠透明带糖蛋白2(ZP2)酶**测试剂盒(ELISA试剂盒) Rattus norvegicus (Rat) Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2)ELISA KIT
猪补体成分3a(C3a)酶**测试剂盒(ELISA试剂盒) Sus scrofa; Porcine (Pig) Complement Component 3a (C3a)ELISA KIT
人早老素1(PSEN1)酶**测试剂盒(ELISA试剂盒) Homo sapiens (Human) Presenilin 1 (PSEN1)ELISA KIT
豚鼠胰岛素(INS)酶**测试剂盒(ELISA试剂盒) Cavia (Guinea pig ) Insulin (INS)ELISA KIT
人肾上腺素能受体β激酶1(ADRβK1)酶**测试剂盒(ELISA试剂盒) Homo sapiens (Human) Adrenergic Receptor Beta Kinase 1 (ADRbK1)ELISA KIT
大鼠骨形成蛋白8A(BMP8A)酶**测试剂盒(ELISA试剂盒) Rattus norvegicus (Rat) Bone Morphogenetic Protein 8A (BMP8A)ELISA KIT
豚鼠内脂素(VF)酶**测试剂盒(ELISA试剂盒) Cavia (Guinea pig ) Visfatin (VF)ELISA KIT
大鼠载脂蛋白A5(APOA5)酶**测试剂盒(ELISA试剂盒) Rattus norvegicus (Rat) Apolipoprotein A5 (APOA5)ELISA KIT
鸡分泌型免疫球蛋白A(sIgA)酶**测试剂盒(ELISA试剂盒) Chicken (Gallus) Secretory Immunoglobulin A (sIgA)ELISA KIT
大鼠组织蛋白酶S(CTSS)酶**测试剂盒(ELISA试剂盒) Rattus norvegicus (Rat) Cathepsin S (CTSS)ELISA KIT
小鼠白介素7(IL7)酶**测试剂盒(ELISA试剂盒) Mus musculus (Mouse) Interleukin 7 (IL7)ELISA KIT
小鼠组织蛋白酶B(CTSB)酶**测试剂盒(ELISA试剂盒) Mus musculus (Mouse) Cathepsin B (CTSB)ELISA KIT
小鼠肿瘤坏死因子受体超家族成员17(TNFRSF17)酶**测试剂盒(ELISA试剂盒) Mus musculus (Mouse) Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17)ELISA KIT
小鼠成纤维细胞生长因子21(FGF21)酶**测试剂盒(ELISA试剂盒) Mus musculus (Mouse) Fibroblast Growth Factor 21 (FGF21)ELISA KIT
人神经降压素受体2(NTSR2)酶**测试剂盒(ELISA试剂盒) Homo sapiens (Human) Neurotensin Receptor 2 (NTSR2)ELISA KIT
人促微管蛋白聚合蛋白(TPPP)酶**测试剂盒(ELISA试剂盒) Homo sapiens (Human) Tubulin Polymerization Promoting Protein (TPPP)ELISA KIT
豚鼠胰岛素(INS)酶**测试剂盒(ELISA试剂盒) Cavia (Guinea pig ) Insulin (INS)ELISA KIT
